parp inhibition olaparib (Cayman Chemical)
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Parp Inhibition Olaparib, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parp+inhibition+olaparib/parp+inhibitor+olaparib/pm37116497-279-1-4
Average 90 stars, based on 1 article reviews
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Inhibition:Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer. Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling. Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin). Polymer:Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer. Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling. Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin). Fluorescence:Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer. Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling. Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin). Western Blot:Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer. Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling. Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin). In Vitro:Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer. Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling. Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin). Recombinant:Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer. Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling. Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin). Transfection:Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer. Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling. Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin). Plasmid Preparation:Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer. Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling. Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin). Multiplex sample analysis:Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer. Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling. Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin). Over Expression:Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer. Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling. Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin). |
