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parp inhibition olaparib  (Cayman Chemical)


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    Structured Review

    Cayman Chemical parp inhibition olaparib
    Parp Inhibition Olaparib, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/parp+inhibition+olaparib/parp+inhibitor+olaparib/pm37116497-279-1-4
    Average 90 stars, based on 1 article reviews
    parp inhibition olaparib - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Inhibition:

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
    Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer.

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
    Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin).

    Polymer:

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
    Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer.

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
    Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin).

    Fluorescence:

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
    Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer.

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
    Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin).

    Western Blot:

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
    Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer.

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
    Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin).

    In Vitro:

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
    Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer.

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
    Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin).

    Recombinant:

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
    Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer.

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
    Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin).

    Transfection:

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
    Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer.

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
    Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin).

    Plasmid Preparation:

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
    Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer.

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
    Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin).

    Multiplex sample analysis:

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
    Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer.

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
    Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin).

    Over Expression:

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
    Article Snippet: After gel extraction (GenElute Gel Extraction Kit, Sigma-Aldrich, USA) the amplicons were sequenced using the respective PCR primer.

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
    Article Snippet: For Hoechst presensitization (live-cell microscopy experiments), growth medium was aspirated and replaced with fresh medium containing 0.3 mg/mL Hoechst 33342 for 1 h at 37 C. Immediately prior to imaging, growth mediumwas replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) or Molecular Probes Live Cell Imaging Solution (Invitrogen) supplemented with 20% fetal bovine serum, 2 mM glutamine, 100 mg/mL penicillin and 100 U/mL streptomycin).



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    Sensitivity towards <t>PARP</t> <t>inhibition</t> and expression of DNA repair factors. (A) Exponentially growing cells were seeded at low, defined numbers and, on the next day, treated with olaparib as indicated. After one week, the medium was exchanged and the cells were incubated without the inhibitor until the formation of colonies. Colored curves represent group means. (B) Expression of DNA repair factors in HPV+ and HPV− HNSCC cell lines as determined by Western blot analysis from exponentially growing cells. Note that TRIP12 expression was also tested but no interpretable results were obtained. (C) Quantification and comparison of DNA repair factors by HPV status. Individual expression is depicted as normalized to the average expression of the whole cohort (dotted line). An asterisk indicates significance (p <0.05, two-tailed Student’s t-test). (D) Association of ATM expression and cell survival in 5 HPV+ and 5 HPV− HNSCC cell lines at 6 Gy as reported previously by us .
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    Selleck Chemicals parp 1 inhibition
    Sensitivity towards <t>PARP</t> <t>inhibition</t> and expression of DNA repair factors. (A) Exponentially growing cells were seeded at low, defined numbers and, on the next day, treated with olaparib as indicated. After one week, the medium was exchanged and the cells were incubated without the inhibitor until the formation of colonies. Colored curves represent group means. (B) Expression of DNA repair factors in HPV+ and HPV− HNSCC cell lines as determined by Western blot analysis from exponentially growing cells. Note that TRIP12 expression was also tested but no interpretable results were obtained. (C) Quantification and comparison of DNA repair factors by HPV status. Individual expression is depicted as normalized to the average expression of the whole cohort (dotted line). An asterisk indicates significance (p <0.05, two-tailed Student’s t-test). (D) Association of ATM expression and cell survival in 5 HPV+ and 5 HPV− HNSCC cell lines at 6 Gy as reported previously by us .
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    Image Search Results


    Sensitivity towards PARP inhibition and expression of DNA repair factors. (A) Exponentially growing cells were seeded at low, defined numbers and, on the next day, treated with olaparib as indicated. After one week, the medium was exchanged and the cells were incubated without the inhibitor until the formation of colonies. Colored curves represent group means. (B) Expression of DNA repair factors in HPV+ and HPV− HNSCC cell lines as determined by Western blot analysis from exponentially growing cells. Note that TRIP12 expression was also tested but no interpretable results were obtained. (C) Quantification and comparison of DNA repair factors by HPV status. Individual expression is depicted as normalized to the average expression of the whole cohort (dotted line). An asterisk indicates significance (p <0.05, two-tailed Student’s t-test). (D) Association of ATM expression and cell survival in 5 HPV+ and 5 HPV− HNSCC cell lines at 6 Gy as reported previously by us .

    Journal: Frontiers in Oncology

    Article Title: A Lack of Effectiveness in the ATM-Orchestrated DNA Damage Response Contributes to the DNA Repair Defect of HPV-Positive Head and Neck Cancer Cells

    doi: 10.3389/fonc.2022.765968

    Figure Lengend Snippet: Sensitivity towards PARP inhibition and expression of DNA repair factors. (A) Exponentially growing cells were seeded at low, defined numbers and, on the next day, treated with olaparib as indicated. After one week, the medium was exchanged and the cells were incubated without the inhibitor until the formation of colonies. Colored curves represent group means. (B) Expression of DNA repair factors in HPV+ and HPV− HNSCC cell lines as determined by Western blot analysis from exponentially growing cells. Note that TRIP12 expression was also tested but no interpretable results were obtained. (C) Quantification and comparison of DNA repair factors by HPV status. Individual expression is depicted as normalized to the average expression of the whole cohort (dotted line). An asterisk indicates significance (p <0.05, two-tailed Student’s t-test). (D) Association of ATM expression and cell survival in 5 HPV+ and 5 HPV− HNSCC cell lines at 6 Gy as reported previously by us .

    Article Snippet: PARP inhibition was performed using olaparib (Selleckchem) at the doses indicated.

    Techniques: Inhibition, Expressing, Incubation, Western Blot, Comparison, Two Tailed Test